Phis-sumo vector

Author: hrhj

August undefined, 2024

Webb11 jan. 2016 · Hexahistidine small ubiquitin-like modifier (SUMO) tagged constructs were generated by cloning the above sequences into a pHis-SUMO vector (Senturia et al. … Webb25 okt. 2012 · SUMO (smallubiquitinlikemodifier 是一种小分子泛素样修饰蛋白 ,广泛存在于各种真核细胞中,参与调节细胞凋亡、信号转导、RNA 转录、蛋白的核质运输以及细胞周 …

pHIS-parallel1 vector map and sequence

WebbpET-28a (+) Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28b (+) or pET-28c (+). Sequence … Webb中文名称： His和SUMO双标签质粒英文名称： pET-N-His-PreScission-SUMO 产品规格： 1μg 100μg 发货周期： 1～3天产品价格：询价本产品仅可用于科研实验，严禁用于其他 … day spring words for the heart ornament

GitHub - HIT-ImmunologyLab/PHISDetector

Webb1 dec. 2009 · The entire resulting cDNAs were sequenced to confirm the site-directed mutations. pFLAG-Sumo-2 and pHis-Sumo-2 cDNAs were constructed by inserting the … WebbNovelMechanismofHeminCapturebyHbp2,the Hemoglobin-bindingHemophorefromListeria monocytogenes* … Webbvariable. A vector xi 2Rm can be assigned one of four disjoint point sets y1, y2, y3 or y4 within an m-dimensional feature space. xi 2Rm is the ith training data row and yi … g clef free

pET-N-His-PreScission-SUMO(原核表达质粒)(D2918-1μg)

Webb第1 页共2 页 Y187-pHis2YeastOne-HybridMediaKit使用说明书产品编号：YM1010（序号1-8），YM1011（YM1010+SL0930）储存温度：序号1-8常温保存；序号9需-20℃储存， … WebbPhis2 1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions … g clef clockWebb26 maj 2024 · 载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically … dayspring xmas cards 2021

"Webbencoding amino acids 1-96 of SUMO-1 (lacking glycine 97 creating a SUMO-1 that is not susceptible to proteolytic removal by isopeptidases) was fused in frame to the amino … " - Phis-sumo vector

Phis-sumo vector

Cloning in the pETM11-Sumo3-eGFP vector – Protein Expression …

WebbpGADT7 AD is a yeast two-hybrid expression vector that is designed to express a fusion protein between the GAL4 activation domain (AD; amino acids 768–881) and a protein of interest. The fusion protein is expressed at high levels from the full-length ADH1 promoter. The fusion protein also contains a hemagglutinin (HA) epitope tag. http://wolfson.huji.ac.il/purification/PDF/Tag_Protein_Purification/SUMO/LifeSensors_SUMOstarInsect.pdf

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WebbWelcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free … WebbWe have a modified pETM11 vector for the expression of SUMO3 fusion proteins available. This vector contains an optimized synthetic human sumo3 gene with appropiate …

http://www.bioon.com.cn/doc/showarticle.asp?newsid=96627 http://www.coolaber.com/usr/2160/File/F/F4671.pdf

WebbpET SUMO (linearized) Linearized bacterial vector with 3'-T overhangs for TA cloning and inducible expression of an N-terminally 6xHis- and SUMO-tagged protein. Sequence … Webb8 aug. 2024 · For the GST pull-down assay, full-length GI was cloned into the pHis-SUMO vector, and the sequence encoding the cytoplasmic tail of SOS1 (residues 453 to 1,146) …

WebbDownload PHIS database for standalone from PHISDetector webserver and check MD5 Due to the large size of the database file (~100G), we recommend using -c (continue) …

Webb2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and … g clef gifWebbVector type Bacterial Expression Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number Low Copy … g clef is also calledWebb27 feb. 2024 · However, removing SUMO is a labor- and time-consuming process. To enhance this system, here we show the construction of a bicistronic vector that allows … days prior meaningWebbChampion Pet Sumo Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … dayspring words of encouragementWebbThe pSUMO vector is a low copy plasmid with kanamycin selection. ( b ) The DNA sequence encoding the SUMO tag. The restriction digestion site of enzyme BsaI is … g clef namesWebbSmt3, hereby referred to as SUMO, is a member of a ubiquitin-like protein family that regulates several cellular processes including apoptosis, nuclear transport, and cell cycle … dayspring worshipWebbTumor-Linked Macrophages Promote HCC Development by Mediating the CCAT1/Let-7b/HMGA2 Signaling Pathway dayspring word of the year 2023