SpletProcedure: Staphylococci isolated from dogs with pyoderma were tested for susceptibility to methicillin by use of a standard disk diffusion test with oxacillin disks. The DNA extracted from the isolates was tested for the mecA gene that encodes the penicillin-binding protein 2a (PBP2a) by use of a polymerase chain reaction (PCR) assay. Splet20. maj 2024 · By testing a panel of ten different β-lactams, we conclude that there is also a great variation in the level of protection conferred by PBP2a. Expression of PBP2a resulted in an only fourfold increase in minimum inhibitory concentration (MIC) for imipenem, while a 32-fold increase in MIC was observed for cefaclor and cephalexin.
Impacts of NaHCO3 on β-Lactam Binding to PBP2a Protein …
SpletAnti-PBP2a McAb (17C8) (1.1 μg/μl in PBS buffer, pH=7.4) ... PCR and latex agglutination test. PCR detected only 37 mecA positive isolates within 62 clinical isolates of S. aureus. LFIA and latex agglutination had similar results to PCR. In this study, the accuracy of PCR and LFIA detection was the same. And similar to our study, these ... SpletOxacillin (1 mu g) disc diffusion and agar dilution method were used. The isolates were also subjected to latex agglutination test for detection of PBP2a and multiplex PCR to detect … steam charts lawbreakers
Laboratory Testing MRSA CDC
SpletThe mecA gene encodes penicillin-binding protein 2a (PBP2a), which is a PBP that has a low affinity for beta-lactam antibiotics. Bacteria expressing this gene can maintain cell … Splet02. okt. 2024 · Staphylococcal resistance to oxacillin/methicillin occurs when an isolate produces an altered penicillin-binding protein, PBP2a, which is encoded mainly by the mecA gene; an additional test detecting oxacillin/methicillin resistance is the latex agglutination assay using the anti-PBP2a monoclonal antibodies and/or direct mecA gene detection ... SpletAbbott™ Clearview™ PBP2a Culture Colony Test. The Clearview™ PBP2a SA Culture Colony Test can provide results up to 24 hours sooner than traditional methods in isolates of S. aureus. Supplier: Abbott™ 893000. Catalog No. 23-029-985. $379.50 / Pack of 25. Qty Check Availability. steam charts empyrion