Flow cytometry protocol for thp-1 cell
WebJun 1, 2024 · This protocol provides instructions to perform a high-throughput small molecule screen using flow cytometry analysis of THP-1 cells, a human monocytic leukemia cell line. We describe a … WebFeb 3, 2024 · Single-cell preparation from different organs, staining of cells with fluorophore-conjugated antibodies, and analysis of the stained cells using flow cytometry are as described 51. Briefly, cells ...
Flow cytometry protocol for thp-1 cell
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WebPrepare your cell suspensions for Flow Cytometry. Although most flow cytometry experiments involve labeling populations of cells that are relatively abundant, the number of cells required will vary depending upon the rarity of your cells. In general, researchers will stain between 1 x 10 5 and 1 x 10 6 cells per sample. WebFlow Cytometry Blocking Controls Fc receptors are found on monocytes, macrophages, dendritic cells and B cells. ... have been developed which, when added to a staining protocol, can ensure that only antigen specific …
Web5 Collect 2 million cytotoxic T cells per sample (i.e. per well) from an on-going culture 6 Spin them down at 350 x gfor 00:05:00 at 4 °C and re-suspend them in fresh media at 1 million per mL concentration 7 Supplement T cells with 200 IU/mL rIL2 8 Aspirate the culture media from each of the 24-well-plate wells that contain a sample. Try to aspirate as much as … WebIntracellular Staining Flow Cytometry Output Using Detergents to Permeabilize Mobile; Centrifuge cells as by Step 3 and resuspend include appropriate volume in Flow Cytometry Coloring Buffer press buffer of choice so that the final cellular concentration is 1 x 10 7 cells/mL (other cell concentrations may been reasonably for different experiments)
WebPublished protocols using the promonocytic cell line THP-1 have tended to result in cells that closely resemble classically-activated macrophages, differentiated in IFNγ and LPS. … WebFor the detection of the viability of Jurkat and THP-1 cells, an MTT assay was employed as previously described . Briefly, both Jurkat and THP-1 cells in the logarithmic growth phase were seeded at a density of 1 × 10 5 cells/well on a 24-well plate and treated with various concentrations (40, 20, 10, 5 and 2.5 µM) of each compound. After the ...
WebFlow Cytometry Reagents. Clinical Diagnostics; ... Single-color flow cytometric analysis of Ig Isotype control staining on THP-1 cells. THP-1 cells were incubated at room …
WebFlow cytometry (FACS) tint protocol (Cell surface staining) Harvest, wash the cells (single fuel suspension) and adjust cell number to a concentration out 1-5x106 cells/ml in ice cold FACS Buffer (PBS, 0.5-1% BSA or 5-10% FBS, 0.1% NaN3 sodium azide*). ... same day. For extended recording (16 hr) in well when for greater flexural in planning ... rcw post-secondary supportWebJan 21, 2024 · Affiliation Flow Cytometry Laboratory, Botucatu Medical School - UNESP, Botucatu, Brazil ⨯ . ... under protocol 2.018.399. THP-1 culture and differentiation. THP-1 cells were cultured in 25 cm 2 sterile … rcw power of trusteeWebPublished protocols using the promonocytic cell line THP-1 have tended to result in cells that closely resemble classically-activated macrophages, differentiated in IFNγ and LPS. However, no protocol, to date, has fully recapitulated polarization of THP-1 to the M(IL-4) or M(IL-10) macrophage phenotypes seen when human monocyte-derived ... rcw properties tim willardWebOct 1, 2024 · Cell yields resulting from THP-1 differentiation in plates (pDMs) and differentiation in tubes (tDMs) were compared by flow cytometry. Cells were … rcw privileged communicationsWebMar 9, 2015 · 1.3 For suspension cells, centrifuge to obtain a cell pellet and aspirate the supernatant. Resuspend the cells gently in prewarmed (37°C) probe-containing medium. Incubate the cells for 30 minutes to 2 hours under growth conditions appropriate for the particular cell type (see note above regarding internalization rate of these probes). Re ... rcw possession of controlled substance methWebJul 14, 2024 · Flow cytometry analysis of treated THP-1 cells was performed for CD68, CD86, and CD206 markers to evaluate M0, M1, and M2 macrophages polarization, respectively. AGS cells were assessed for apoptosis and necrosis by flow cytometry after labeling with Annexin V-FITC and propidium iodide. rcw privacy actWebOur flow cytometry protocols cover matters like sample prep of mouse and rat leucocytes, indirect staining of mononuclear total, also reducer nonspecific paint with Fc Block. Skip for main content Miss go navigation. Order Lookup. … how to speed up a slow gut