Determination of aconitase activity
WebJun 23, 2024 · Prior studies indicated that H129 is the only histidine ligand involved in the control of the iron donation capability of YtfE, as its replacement for a leucine residue produces a protein unable to restore the aconitase activity . Interestingly, H129 forms an H-bond with E159 (H129 Nδ1 …E159 Oε1 2.66 Å). WebDetermination of Aconitase Activity—For the determination of the aconitase activity, cells from the CGXII main culture (∼25 ml) were harvested with ∼25 g of crushed ice (precooled to -20 °C) by centrifugation at 4,000 × g for 5 min. The cell pellet was resuspended in 900 μl of Tris-HCl (90 m m g; 4 °C), the supernatant was used ...
Determination of aconitase activity
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WebJul 9, 2004 · Aconitase, a Krebs-cycle enzyme that converts citrate to isocitrate, belongs to the family of iron-sulfur–containing dehydratases whose activities depend on an intact cubane [4Fe-4S] 2+ cluster (1, 2).The purified enzyme is highly susceptible to oxidant-induced inactivation due to release of the solvent-exposed Fe-α and formation of a [3Fe … WebThe aconitase activity was significantly depressed in the 2% O2 group (*P ⬍ 0.03, n ⫽ 6/group). ... Determination of the mechanism of free radical generation in damage in the presence of cyanosis: a new concept of controlled cardiac human aortic endothelial cells exposed to anoxia and reoxygenation. ...
WebAug 1, 2005 · Unanticipated, however, was the reduced expression of the tricarboxylic acid (TCA) cycle gene citB (encoding aconitase), resulting in the loss of aconitase activity and preventing the ... WebApr 14, 2024 · Plants of the genus Scutellaria (Lamiaceae) have a wide variety of bioactive secondary metabolites with diverse biological properties, e.g., anti-inflammatory, antiallergenic, antioxidant, antiviral, and antitumor activities. The chemical composition of the hydroethanolic extracts, obtained from dried plants of S. incarnata, S. coccinea, and …
WebApr 10, 2024 · This piece explains an activity that allows for the simple and accurate determination of the heat of vaporization, ΔH vap, of water at 100°C, and ultimately the approximate strength of a hydrogen bond in boiling water, in kJ·mol –1. The vaporization of water is an endothermic process represented by Equation 1: WebAconitase 2 ( ACO2 ) encodes the mitochondrial aconitase (ACO2), an enzyme catalyzing interconversion of citrate into isocitrate in the Krebs cycle. ACO2 mutations have been initially associated with infantile cerebellar-retinal degeneration combining optic atrophy, retinal degeneration, severe encephalopathy, epilepsy, and cerebellar ataxia1–3; …
WebThe aconitase activity of a sample may be determined by the following equation: Aconitase Activity = B Sample Dilution Factor (milliunit/mL) T V B = Amount (nmole) of …
WebDetermination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease Pedro M. Quirós Abstract Mitochondrial aconitase is a reversible enzyme that catalyzes the … birthday celebration images clip artWebJun 1, 2015 · The effect of pH on aconitase activity was studied in 100 mM tris–HCl buffer containing 40 mM citrate or 4 mM d, l-isocitrate. Determination of K i was performed by plotting the graphs of dependence of 1/v from the concentration of inhibitor [i] at two fixed concentrations of substrate (citrate) (Dixon, 1953). danish pronounce peugeotWebThus, aconitase activity is used as an indicator of levels of intracellular H 2 O 2 and its oxidative damage to cellular components. For example, substantially decreased … danish productsWebBuy Sodium monofluoroacetate, 99% (CAS No. 62-74-8) from Smolecule. Molecular Formula: C2H2FO2.Na C2H2FO2.Na CH2FCOONa C2H2FNaO2. Molecular Weight: 100.02 g/mol. Introduction Sodium monofluoroacetate, also known as 1080, is a highly toxic chemical compound that has been categorized as a Schedule 7 poison in Australia and … danish pronounce babarWebSep 17, 2024 · These intermediates are capable of oxidizing the cluster, which leads to iron release and consequent loss of the catalytic activity of aconitase. As the reaction of the Fe-S cluster with O 2 •-is fast (∼10 7 M-1 s-1), quite specific, and reversible in vivo, quantification of active aconitase has been used to evaluate O 2 •-formation in cells. danish pronounce mercedes-benzWebEscherichia coli possesses two aconitases, a stationary-phase enzyme (AcnA), which is induced by iron and oxidative stress, and a major but less stable enzyme (AcnB), synthesized during exponential growth. In addition to the catalytic activities of the holo-proteins, the apo-proteins function as post-transcriptional regulators by site-specific … birthday celebration ideas sydneyWebSomething that is curious about aconitase is the fact that it uses iron as the inorganic substituent. Mutanogenic studies on aconitase have found substituting the iron ions with … danish pronounce teletubbies